|
KMID : 1007020070050010011
|
|
Korean Soceity of Osteroporosis
2007 Volume.5 No. 1 p.11 ~ p.19
|
|
|
The Relationship Between Osteoprotegerin (OPG)-receptor Activator of NF-kB Ligand (RAnKL) Gene Ploymorphism and Their Production by Whole Blood Cells
|
|
Kim Yong-Jin
Suh Chang-Suk
Kim Jung-Gu
Kim Seok-Hyun
Choi Young-Min
Moon Shin-Yong
Kim Hoon
Chae Soo-Jin
Park Kyoung-Ah
|
|
|
Abstract
|
|
|
Objective: To investigate the association between polymorpjhisms of osteoprotegerin(OPG), and receptor activator of nuclear factor-[kappa]B ligand(RANKL) genes and production of OPG, and soluble RANKL (sRANKL) by whole blood cellsin Korean postmenopausal women.
Methods: The OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 267 Korean postmenopausal women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured.
Results: The productions of OPG, sRANKL and the ratio of RANKL×1000/OPG by lipopolysaccharide-stimulated whole blood cells were not different according to the OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms. No significant differences in the productions of OPG, sRANKL and the ratio of RANKL×1000/OPG were noted among the haplotype genotypes of OPG gene T245G and G1181C polymorphisms. There were no significant differences in the productions of OPG, sRANKL and the ratio of RANKL×1000/OPG among combined genotypes of OPG gene G1181C and RANKL gene rs2277438 polymorphisms.
Conclusion: The OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms do not affect the production of OPG, and sRANKL by lipopolysaccharide-stimulated whole blood cells.
|
|
|
KEYWORD
|
|
|
Whole blood cells, Osteoprotegerin (OPG), Receptir activator of nuclear factor-[kappa] Bligand (RANKL), Gene polymorphism
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|